Principles and Technique

Total Lectures


Mod-01 Lec-01 Introduction to Proteomics Course


Mod-01 Lec-01 Introduction to Proteomics Course
Mod-02 Lec-02 Central Dogma: Basics of DNA, RNA, Proteins
Mod-03 Lec-03 Genomics and Transcriptomics: Why proteomics?
Mod-04 Lec-04 Proteins: Amino acids and structural levels of proteins
Mod-05 Lec-05 Proteins: Folding and misfolding
Mod-06 Lec-06 Protein Purification and Peptide Isolation using Chromatography
Mod-07 Lec-07 Enzymes: Basic concepts, Catalytic and Regulatory strategies
Mod-08 Lec-08 Proteomics and Systems Biology
Mod-09 Lec-09 Sample preparation for proteomics applications
Mod-10 Lec-10 Sample preparation for proteomics applications: Serum and bacterial proteome
Mod-11 Lec-11 Sample preparation for proteomics applications
Mod-12 Lec-12 Gelbased Proteomics
Mod-13 Lec-13 Gel-based Proteomics Two-dimensional electrophoresis(continued)
Mod-14 Lec-14 Gel-based Proteomics Two-dimensional electrophoresis Workflow
Mod-15 Lec-15 Two-dimensional electrophoresis: Image processing and data analysis
Mod-16 Lec-16 Two-dimensional difference gel electrophoresis (2D DIGE)
Mod-17 Lec-17 Difference gel electrophoresis (continued) Discussion and data analysis
Mod-18 Lec-18 Applications of two dimensional electrophoresis
Mod-19 Lec-19 Applications of 2-DE and DIGE
Mod-20 Lec-20 Fundamentals of mass spectrometry
Mod-21 Lec-21 Matrix assisted laser desorption/ionization-Time of Flight (MALDI-TOF)
Mod-22 Lec-22 Liquid chromatography-Mass spectrometry (LC-MS/MS)
Mod-23 Lec-23 Hybrid-MS/MS Configurations
Mod-24 Lec-24 Quantitative Proteomics: Stable Isotope Labeling by Amino Acids in Cell Culture(SILAC)
Mod-25 Lec-25 Quantitative Proteomics: iTRAQ and TMT
Mod-26 Lec-26 Interactomics: Yeast Two-Hybrid Immunoprecipitation Protein microarrays
Mod-27 Lec-27 Microarray workflow: Label-based detection techniques
Mod-28 Lec-28 Microarray related concepts: Recombinational cloning Cell-free expression
Mod-29 Lec-29 Cell-free synthesis based protein microarrays
Mod-30 Lec-30 Generating Protein Microarrays: Focus on Nucleic Acid Programmable Protein Array
Mod-31 Lec-31 Microarray work-flow: Image scanning and processing
Mod-32 Lec-32 Microarray work-flow: Data analysis
Mod-33 Lec-33 Applications of protein microarrays
Mod-34 Lec-34 Applications of cell free protein microarrays
Mod-35 Lec-35 Label-free techniques: SPR and SPRi
Mod-36 Lec-36 Label-free techniques: SPRi, Ellipsometry, Interference
Mod-37 Lec-37 Surface Plasmon Resonance: Biacore SPR and data analysis
Mod-38 Lec-38 Nanotechniques in proteomics
Mod-39 Lec-39 Detection System: Diffraction-based biosensors
Mod-40 Lec-40 Proteomics: Advances and Challenges
OER.UoCh.Edu.Pk (University of Chitral Open Educational Resources).

In the wake of the global emergency on Novel Coronavirus (COVID-19), the universities of Pakistan are planning to go online under the aegis of the Higher Education Commission of Pakistan (HEC). The University of Chitral has launched a comprehensive open learning knowledge hub to assist the faculty and students both at home and abroad to learn through the web. This includes a wide range of learning resources including free audio-visual lectures from the top universities and institutes of the world.